Detection of Rickettsia spp. in ticks fixed on people in Castilla y León
Abstract
Ticks are hematophagous parasites of terrestrial vertebrates with great importance because they are transmitters of pathogenic agents. The aim of this study consisted of the analysis of ticks fixed on people in Castilla y Leon in summer, more specifically the detection of rickettsiosis. We recived 709 ticks removed to people in the diferents health center or hospital in this community. In the laboratory was carried out its identification and extraction of DNA by a commercial kit Genomic DNA from Tissue. Then, we performed an individualized PCR to each sample in order to know if they are carriers of Rickettsia spp. To see the results of the PCR, was made an electrophoresis gel agarose, which is displayed with a transilluminator BioDoc-it2 Imaging Systems. Finally, the positive products were purified and quantified. Of the samples analyzed, for genes gltA and OmpA, were obtained 67 positive (9.44%), presenting greater prevalence in the provinces of Avila and Burgos. Therefore, this study concludes that the growing interest in tick-borne diseases is indisputable, and the data obtained are very important in guiding measures to control and prevent these diseases.
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Brouqui P et al. Spotted fever rickettsioses in southern and eastern Europe. FEMS Immunol Med Microbiol. 2007; 49(1):2-12.
Cdc.gob [Internet]. Estados Unidos: Departamet of Health & Human Services; [actualizado 31 dic. 2017]. Disponible en: https://www.cdc.gov/dpdx/ticks/index.html.
Estrada-Peña A. Orden Ixodida: Las garrapatas. Revista IDE@-SEA. 2015; 13:1-15.
Gil-Collado J et al. Claves para la identi?cación de los Ixodoidea españoles (adultos). Revista Ibérica de Parasitolología. 1979; 39:107-118.
Herrero QJA et al. Infecciones por rickettsias y fiebre. Medicine. 2010; 10(57):3881-3888.
Parola P et al. Tick-borne rickettsioses around the world: emerging diseases challenging old concepts. J Clin Microbiol 2005; 18(4):719-756.
Quintero JC et al. Ecoepidemiología de la infección por rickettsias en roedores, ectoparásitos y humanos en el noroeste de Antioquia, Colombia. Biomedica. 2013; 33(1):38-35.
Regnery RL et al. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol. 1991; 173(5): 1576-1589.
Remesar S et al. Tick species diversity and population dynamics of Ixodes ricinus in Galicia (north-western Spain). Ticks Tick-Borne Dis. 2019; 10:132-137.
Roux V et al. Differentiation of spoted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA. J Clin Microbiol. 1996; 34:2058-2065.
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