Liposomes isolation and characterization by microencapsulation
Abstract
Liposomes are microscopic vesicles composed of concentric lipid bilayers entrapping aqueous compartments. Proteins and lipids are among biocompatible and biodegradable materials used for microencapsulation, being albumin an interesting protein due to its role as a drugs carrier.
The aim of the study was to encapsulate ciprofloxacin loaded liposomes into Albusomes® a novel pharmaceutical vehicle based on albumin coated liposomes.
The vesicle preparation was carried out using the lipid hydration method and sonication method using a mixture of egg phosphatidylcholine (EPC), diethyldodeccyiamonium (DDA) and cholesterol (Ch).
The liposomes were encapsulated with bovine serum albumin by induced flocculation. The pellet, containing the albumin coated liposomes with ciprofloxacin trapped into water vesicle core and albumin coating layers, was isolated by centrifugation. Concentration of ciprofloxacin was quantified in the supernatant and the absence of lipid vesicles was confirmed. The proposed vehicle (Albusomes®) showed to be able to encapsulate liposomes with a high drug entrapment efficacy (86.0 ± 5.0% and 93.52±3.94%). for lipid hydration method and sonication method, respectively.
- Referencias
- Cómo citar
- Del mismo autor
- Métricas
Clares, B. Tesis doctoral. Sistemas de Transporte y Liberación de Fármacos de Aplicación Tópica: Liposomas multilamilares portadores de Acetonido de Triamcinolona. Universidad de Granad, España 2003.
De Jesús Valle M J, Sánchez Navarro A. Liposomes Prepared in Absence of Organic Solvents: Sonication Versus Lipid Film Hydration Method. Curr Pharm Anal, 2015; 11(2): 86-91.
Ferreira M, Gameiro P. Ciprofloxacin Metalloantibiotic: An effective antibiotic with an influx route strongly dependent on lipid interaction? J Membr Biol. 2015: 125-136.
Gala RP, Khan I, Elhissi AM, Alhnan MA. A comprehensive production method of self-cryoproted nano-liposome powders. Int J Pharm. 2015;486:153-158.
Hamblin KA,Wong JP, Blanchard JD, Atkins HS. The potential of liposome- encapsulated ciprofloxacin as a tularemia therapy. Front Cell Infect Microbiol. 2014; 4:1-5.
Madni A, Sarfraz M, Ahmad M, Akhtar N, Ahmad S, Tahir N, Ijaz S, et al. Liposomal drug delivery: A versatile platform for challenging clinical applications. J Pharm Sci. 2014; 17(3): 401-426.
Ruozi B, Tosi G, Fomi F, Fresta M, Vandelli MA. Atomic force microscopy and photon correlation spectroscopy: Two techniques for rapid characterization of liposomes. Eur J Pharm Sci. 2005; 25: 81-89.
Torchilin VP. Multifunctional, stimuli-sensitive nanoparticulate systems for drug delivery. Nat Rev Drug Discov. 2014; 13:813-827.
Torchilin VP. Recent advances with liposomes as pharnaceutical carriers. Nat Rev Drug Discov. 2005; 4:145-160.
Zawada ZH. Liposomes from hydrogenated soya lecithin formed in sintered glass pores. Acta Pol Pharm. 2012; 69(1): 107-111.
Most read articles by the same author(s)
- Oscar Delgado-Rubio, María José De Jesus-valle, Amparo Sanchez-Navarro, Design of a liposomal formulation aimed to vaccines administration , FarmaJournal: Vol. 1 No. 1 (2016)
- Jimena Barbero, Amparo Sánchez Navarro, María José de Jesús-Valle, Ergosterol Liposomes in Lyophilized Formulations for Buccal Administration , FarmaJournal: Vol. 9 No. 2 (2024)
- Sara Viruega-Encinas, María José De Jesús-valle, Microencapsulation of antifungal agents in albumin coated liposomes , FarmaJournal: Vol. 2 No. 2 (2017)
Similar Articles
- Juan José Duque Aguilar, María José Jesús Del Valle, Amparo Sánchez Navarro, Microencapsulation of ciprofloxacin in microspheres of albumin and liposomes (Albusomes) , FarmaJournal: Vol. 2 No. 2 (2017)
- Sara Viruega-Encinas, María José De Jesús-valle, Microencapsulation of antifungal agents in albumin coated liposomes , FarmaJournal: Vol. 2 No. 2 (2017)
- Oscar Delgado-Rubio, María José De Jesus-valle, Amparo Sanchez-Navarro, Design of a liposomal formulation aimed to vaccines administration , FarmaJournal: Vol. 1 No. 1 (2016)
- Jimena Barbero, Amparo Sánchez Navarro, María José de Jesús-Valle, Ergosterol Liposomes in Lyophilized Formulations for Buccal Administration , FarmaJournal: Vol. 9 No. 2 (2024)
- Azahara I. Toro, María José de Jesús Valle, Drug delivery from liposomes in different biorelevant media , FarmaJournal: Vol. 6 No. 1 (2021)
- Laura Garrido de Gracia, María José de Jesús Valle, Aránzazu Zarzuelo Castañeda, Amparo Sánchez Navarro, Development of Mucoadhesive Oral Formulations Based on Resveratrol Liposomes , FarmaJournal: Vol. 10 No. 2 (2025)
- Blanca Calderón García, María José De Jesús Valle, Lyophillization of Liposomes for Pulmonary Administration , FarmaJournal: Vol. 7 No. 2 (2022)
- Salomé Díaz Sánchez, José Martínez Lanao, Clara Isabel Colino Gandarillas, Incorporation of anti-infectious agents on metallic nanoparticles of gold and chitosan , FarmaJournal: Vol. 3 No. 2 (2018)
- Laura Pérez-Sánchez, Alfredo Ginés Casanova, Ana Isabel Morales, Nephrotoxicity biomarkers able to identify oncological patients at risk of developing kidney damage , FarmaJournal: Vol. 4 No. 2 (2019)
- Alicia Abril, Javier Tascón, Laura Vicente-Vicente, Alfredo G. Casanova, Marta Prieto, Ana I. Morales, Characterization of Renal Damage Associated with Oncologic Immunotherapy , FarmaJournal: Vol. 8 No. 1 (2023)
You may also start an advanced similarity search for this article.