Adaptación de la PFK1 en células nerviosas de ratón en cultivo primario a las concentraciones fisiológicas de oxígeno

Abstract

Glycolysis links the need to obtain energy with metabolism, moreover, it’s the previous metabolic pathway for the mitochondria to produce the necessary energy. The main glycolysis regulatory enzyme is phosphofructokinase-1 (PFK1) whose regulation is complex. This enzyme has three isoforms PFK1-L, PFK1-M and PFK1-C whose expression level is specific to each tissue. The objective of this work is to analyze the effect that physiological concentrations of O₂, which are 5?%, have on the maximum activity and the total amount of PFK1 as a regulating enzyme of glycolysis. For this, neuron and astrocyte crop were used in monocrop and in cocrop with O₂ concentrations of 21?% and 5?%. The results obtained show that astrocytes presents an activity between 4-5 times greater than neurons both in monocrop and in cocrop, moreover, their presence causes greater activity in neurons at both 21?% and 5?% O₂. The increased activity in neuron monocrop is related to a higher expression of PFK1-L, while that of cocrop may be due to increased expression of PFK1-L and PFK1-M.