Adaptation of Pfk1 in Mouse Nerve Cells in Primary Crop to Physiological Concentrations of Oxygen
Abstract Glycolysis links the need to obtain energy with metabolism, moreover, it’s the previous metabolic pathway for the mitochondria to produce the necessary energy. The main glycolysis regulatory enzyme is phosphofructokinase-1 (PFK1) whose regulation is complex. This enzyme has three isoforms PFK1-L, PFK1-M and PFK1-C whose expression level is specific to each tissue. The objective of this work is to analyze the effect that physiological concentrations of O2, which are 5?%, have on the maximum activity and the total amount of PFK1 as a regulating enzyme of glycolysis. For this, neuron and astrocyte crop were used in monocrop and in cocrop with O2 concentrations of 21?% and 5?%. The results obtained show that astrocytes presents an activity between 4-5 times greater than neurons both in monocrop and in cocrop, moreover, their presence causes greater activity in neurons at both 21?% and 5?% O2. The increased activity in neuron monocrop is related to a higher expression of PFK1-L, while that of cocrop may be due to increased expression of PFK1-L and PFK1-M.
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Herrero-Mendez A. et al. The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C-Cdh1. Nat Cell Biol. 2009; 11(6):747-752.
Mhaskar Y, Dunaway GA. Alteration of PFK subunit protein, synthesis, and mRNA during neonatal brain development. Elsevier. 1995; 85(1):54-57.
Nelson DL, Coz MM. Lehninger. Principios de bioquímica. 5.ª ed. Estados Unidos: Omega; 2017. pp. 527-538.
Roca P et al. Bioquímica. Técnicas y métodos. 1.ª ed. España: Hélice; 2003. pp. 156-157; 168-170.
Rodwell VW et al. Harper’s. Illustrated biochemistry. 30.ª ed. Estados Unidos: McGraw-Hill; 2009. pp. 168-175.
Sola-Penna M et al. Regulation of mammalian muscle type 6-phosphofructo-1-kinase and its implication for the control of the metabolism. IUBMB Life. 2010; 62(11):791-796.
Tanner LB et al. Four key steps control glycolytic flux in mammalian cells. Cell Syst. 2018; 7(1):49-62e8.
Webb BA et al. The glycolytic enzyme phosphofructokinase-1 assembles into filaments. J Cell Biol. 2017; 216(8):2305-2313.
Martínez Luna, M., & Fernández Sánchez, E. (2021). Adaptation of Pfk1 in Mouse Nerve Cells in Primary Crop to Physiological Concentrations of Oxygen. FarmaJournal, 6(2), 7–15. https://doi.org/10.14201/fj202162715
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