Application of the Strong-LAMP method for the molecular detection of Strongyloides stercoralis in a population of Porto Iguazú, Misiones, Argentina
Abstract Strongiloides stercoralis is a nematode globally distributed and the chief causative agent of human Strongyloidiasis. Chronic infection is clinically asymptomatic, but, it can cause Hyperinfection Syndrome in immunocompromised patients. At present, the lack of a standardized diagnosis generates an underestimation of the prevalence of the disease. Coprological test, considered as the gold-standard, has a very low sensitivity. An alternative is the specific and sensitive LAMP method for the detection of S.stercoralis, the Strong-LAMP. In the present study, it have been used a total of 100 stool samples collected from a population of Porto Iguazú, Misiones (Argentina) and stored on filter paper until analysis. When Strong-LAMP was applied for the detection of Strongyloides spp. DNA, up to 39 samples (39%) were positive, including 5 positive obtained by coprological test, compared to 7 positive samples (7%) obtained by parasitological test. Therefore, Strong-LAMP has demonstrated more sensitive method for the detection of Strongyloides stercoralis in stool samples stored in filter paper than parasitological diagnostic methods and could be used in combination with parasitological methods in endemic areas to establish with greater reliability the real prevalence of the disease.
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Schär F, Trostdort U, Giardina F, Khieu V, Muth S, Vounatsou P et al. Strongyloides stercoralis: Global Distribution and Risk Factors. Plos Neglect Trop D. 2013; 7(7):e2288. https://doi.org/10.1371/journal.pntd.0002288
Buonfrate D, Formenti F, Perandin F, Bisoffi Z. Novel approaches to the diagnosis of Strongyloides stercoralis infection. Clin Microbiol Infect. 2015; 21(6):543-552. https://doi.org/10.1016/j.cmi.2015.04.001
Cevallos W, Fernández-Soto P, Calvopiña M, Buendía-Sánchez M, López-Abán J, Vicente B. et al. Diagnosis of amphimeriasis by LAMPhimerus assay in human stool samples long-term storage onto filter paper. PLoS One. 2018; 13(2):e0192637. https://doi.org/10.1371/journal.pone.0192637
Cevallos W, Fernández-Soto P, Calvopiña C, Fontecha-Cuenca C, Sugiyama H, Sato M. et al. LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples. PLos Negl Trop Dis. 2017; 11(6):e0005672. https://doi.org/10.1371/journal.pntd.0005672
Faust EC, Russell PF, Jung RC. Nematodos fasmidios parásitos del hombre. En: Craig y Faust, editores. Parasitología clínica; 1983. pp. 284-296.
Fernández-Soto P, Sánchez-Hernández A, Gandasegui J, Bajo Santos C, Lopez-Abán J, Saugar JM et al. Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model. Plos Neglect Trop D. 2016; 10(7):e0004836. https://doi.org/10.1371/journal.pntd.0004836
Henríquez-Camacho C, Gotuzzo E, Echevarría J, White AC Jr, Terashima A, Samalvides F et al. Ivermectin versus albendazole or thiabendazole for Strogyloides stercoralis infection. Cochrane Database Syst Rev. 2016; (1):CD007745. https://doi.org/10.1002/14651858.CD007745.pub3
King SE, Mascie-Taylor CG. Strongyloides fuelleborni kellyi and other intestinal helminths in children from Papua New Guinea: associations with nutritional status and socioeconomic factors. PnG. Med. J. 2004; 47:181.
Li Y, Fan P, Zhou S, Zhang L. Loop-mediated isothermal amplification (LAMP): A novel rapid detection platform for pathogens. Microb Pathog. 2017; 107:54-61. https://doi.org/10.1016/j.micpath.2017.03.016
Montes M, Sawhney C, Barros N. Strongyloides stercoralis: there but not seen. Curr Opin Infect Dis. 2010; 23(5):500. https://doi.org/10.1097/QCO.0b013e32833df718
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. https://doi.org/10.1093/nar/28.12.e63
Puthiyakunnon S, Boddu S, Li Y, Zhou X, Wang C, Li J, Chen X. Strongyloidiasis-An Insight into Its Global Prevalence and Management. PLoS Negl Trop Dis. 2014; 8(8):e3018. https://doi.org/10.1371/journal.pntd.0003018
Schär F, Trostdort U, Giardina F, Khieu V, Muth S, Vounatsou P et al. Strongyloides stercoralis: Global Distribution and Risk Factors. Plos Neglect Trop D. 2013; 7(7):e2288. https://doi.org/10.1371/journal.pntd.0002288
Rodríguez Diego, C., Fernández-Soto, P., & Muro Álvarez, A. (2020). Application of the Strong-LAMP method for the molecular detection of Strongyloides stercoralis in a population of Porto Iguazú, Misiones, Argentina. FarmaJournal, 5(2), 73–82. https://doi.org/10.14201/fj2020527382
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