Bio-prospecting for antibiotic producing bacteria from the rhizospheric culturome
Abstract The inappropriate use of antibiotics has increased the number illnesses and deaths because of multidrug resistant microbial caused infections. The objective of this study was to isolate rhizospheric bacteria and evaluate their potential to produce antimicrobial compounds. 418 bacterial colonies were isolated and assayed against bacteria and fungi in order to analyze their antimicrobial potential, finding 114 antimicrobial producing strains. Two of these strains with best antimicrobial spectrum of action were selected for further analyses, and were identified as Bacillus albus and Streptomycs cirratus. Their genomes were sequenced and secondary metabolism pathways were analyzed, finding genetic machinery potentially implicated in the production of new bioactive compounds.
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Adamek M, Spohn M, Stegmann E, Ziemert N. Mining bacterial genomes for secondary metabolite gene clusters. Methods Mol Biol. 2017; 1520:23-47. https://doi.org/10.1007/978-1-4939-6634-9_2
Banin E, Hughes D, Kuipers O.P. Editorial: Bacterial pathogens, antibiotics and antibiotic resistance. FEMS Microbiol Rev. 2017; 41(3):450-452. https://doi.org/10.1093/femsre/fux016
Demain A.L. Pharmaceutically active secondary metabolites of microorganisms. Appl Microbiol Biotechnol. 1999; 52(4):455-463. https://doi.org/10.1007/s002530051546
Horcajada JP, Graus S, Paño-Pardo JR, López A, Oliver A, Cisneros JM et al. Antimicrobial stewardship in Spain: programs for optimizing the use of antibiotics (PROA) in Spanish hospitals. Germs. 2018; 8(3):109-112. https://doi.org/10.18683/germs.2018.1137
Iftime D, Kulik A, Härtner T, Rohrer S, Niedermeyer TH, Stegman E et al. Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365. J Ind Microbiol Biotechnol. 2015; 4(2):277-291. https://doi.org/10.1007/s10295-015-1685-7
Jiménez-Gómez A, Saati-Santamaría Z, Igual JM, Rivas R, Mateos PF, García-Fraile P. Genome insights into the novel species Microvirga brassicacearum, a rapeseed endophyte with biotechnological potential. Microorganisms. 2019; 7(9):354. https://doi.org/10.3390/microorganisms7090354
Kevany BM, Raski DA, Thomas MG. Genetics of zwittermicin a production by Bacillus cereus. Appl Environ Microbiol. 2009; 75(4):1144-1155. https://doi.org/10.1128/AEM.02518-08
Matilla MA, Krell T. Bacterias rizosféricas como fuente de antibióticos. Alianzas y Tendencias. 2017; 2(1):14-21.
Poveda J, Jiménez-Gómez A, Saati-Santamaría Z, Usategui-Martín R, Rivas R, García-Fraile P. Mealworm frass as a potential biofertilizer and abiotic stress tolerance-inductor in plants. Appl Soil Ecol. 2019; 142:110-122. https://doi.org/10.1016/j.apsoil.2019.04.016
Saati-Santamaría Z, López-Mondéjar R, Jiménez-Gómez A, Díez-Mendez A, V?trovský T, Igual JM et al. Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Peudomonas bohemica sp. nov. Front Microbiol. 2018; 9:913. https://doi.org/10.3389/fmicb.2018.00913
Sansinenea E, Ortiz A. Secondary metabolites of soil Bacillus spp. Biotechnol Lett. 2011, 33:1523-1538. https://doi.org/10.1007/s10529-011-0617-5
Wang G, Manns DC, Churey JJ, Worobo RW. Development of a homologous expression system for and systematic site-directed mutagenesis analysis of thurincin H, a bacteriocin produced by Bacillus thuringiensis SF361. Appl Environ Microbiol. 2014; 80(12):3576-3584. https://doi.org/10.1128/AEM.00433-14
Banin E, Hughes D, Kuipers O.P. Editorial: Bacterial pathogens, antibiotics and antibiotic resistance. FEMS Microbiol Rev. 2017; 41(3):450-452. https://doi.org/10.1093/femsre/fux016
Demain A.L. Pharmaceutically active secondary metabolites of microorganisms. Appl Microbiol Biotechnol. 1999; 52(4):455-463. https://doi.org/10.1007/s002530051546
Horcajada JP, Graus S, Paño-Pardo JR, López A, Oliver A, Cisneros JM et al. Antimicrobial stewardship in Spain: programs for optimizing the use of antibiotics (PROA) in Spanish hospitals. Germs. 2018; 8(3):109-112. https://doi.org/10.18683/germs.2018.1137
Iftime D, Kulik A, Härtner T, Rohrer S, Niedermeyer TH, Stegman E et al. Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365. J Ind Microbiol Biotechnol. 2015; 4(2):277-291. https://doi.org/10.1007/s10295-015-1685-7
Jiménez-Gómez A, Saati-Santamaría Z, Igual JM, Rivas R, Mateos PF, García-Fraile P. Genome insights into the novel species Microvirga brassicacearum, a rapeseed endophyte with biotechnological potential. Microorganisms. 2019; 7(9):354. https://doi.org/10.3390/microorganisms7090354
Kevany BM, Raski DA, Thomas MG. Genetics of zwittermicin a production by Bacillus cereus. Appl Environ Microbiol. 2009; 75(4):1144-1155. https://doi.org/10.1128/AEM.02518-08
Matilla MA, Krell T. Bacterias rizosféricas como fuente de antibióticos. Alianzas y Tendencias. 2017; 2(1):14-21.
Poveda J, Jiménez-Gómez A, Saati-Santamaría Z, Usategui-Martín R, Rivas R, García-Fraile P. Mealworm frass as a potential biofertilizer and abiotic stress tolerance-inductor in plants. Appl Soil Ecol. 2019; 142:110-122. https://doi.org/10.1016/j.apsoil.2019.04.016
Saati-Santamaría Z, López-Mondéjar R, Jiménez-Gómez A, Díez-Mendez A, V?trovský T, Igual JM et al. Discovery of phloeophagus beetles as a source of Pseudomonas strains that produce potentially new bioactive substances and description of Peudomonas bohemica sp. nov. Front Microbiol. 2018; 9:913. https://doi.org/10.3389/fmicb.2018.00913
Sansinenea E, Ortiz A. Secondary metabolites of soil Bacillus spp. Biotechnol Lett. 2011, 33:1523-1538. https://doi.org/10.1007/s10529-011-0617-5
Wang G, Manns DC, Churey JJ, Worobo RW. Development of a homologous expression system for and systematic site-directed mutagenesis analysis of thurincin H, a bacteriocin produced by Bacillus thuringiensis SF361. Appl Environ Microbiol. 2014; 80(12):3576-3584. https://doi.org/10.1128/AEM.00433-14
Fernández-Villacorta, N., Mateos, P. F., & Saati-Santamaría, Z. (2020). Bio-prospecting for antibiotic producing bacteria from the rhizospheric culturome. FarmaJournal, 5(2), 43–50. https://doi.org/10.14201/fj2020524350
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