Incorporation of anti-infectious agents on metallic nanoparticles of gold and chitosan
Abstract Resistance to antimicrobial agents is a serious health problem, causing great mortality in the population. This makes very interesting the search for alternatives for the treatment of these infections caused by multiresistant bacteria. One of them could be the use of gold metallic nanoparticles (NPs) together with an anti-infectious agent such as ciprofloxacin which is a broad-spectrum antibacterial drug. The objective of the study was to evaluate the behavior of gold-chitosan (Au-chitosan) NPs compared to gold-citrate (Au-citrate) NPs in the uptake of ciprofloxacin for different incubation conditions. The nanoparticles were synthesized and the anti-infectious agent incorporated at different study concentrations. By ultra-high performance liquid chromatography (UHPLC), the concentrations of ciprofloxacin incorporated into the nanoparticles were estimated. The percentage incorporation of ciprofloxacin was higher for Au-citrate nanoparticles compared to Au-chitosan nanoparticles. However, Au-citrate disadvantage form large aggregates, difficulting its good management. Au-chitosan NPs are more stable and chitosan has antibacterial activities. Thus, gold and chitosan nanoparticles could be a good vehicle for the incorporation of anti-infective agents and may even have the potential to enhance their antimicrobial activity.
- Referencias
- Cómo citar
- Del mismo autor
- Métricas
Al-Qadi S, Remuñán-López C. Nanopartículas metálicas: oro. [Santiago de Compostela]: Universidad de Santiago de Compostela. 2009 [acceso 25 abr 2017] Monografías de la Real Academia Nacional de Farmacia. Disponible en: http://www.analesranf.com/ index.php/mono/article/view/994/0
Burygin G, Khlebtsov B, Shantrokha A, Dykman L, Bogatyrev V, Khlebtsov N. On the Enhanced Antibacterial Activity of Antibiotics Mixed with Gold Nanoparticles. Nanoscale Research Letters. 2009;4(8):794-801.
Fernández Riverón F, López Hernández J, Ponce Martínez LM, Machado Betarte C. Resistencia bacteriana. Rev Cub Med Mil [Internet]. 2003[acceso 25 abr 2017]; 32(1): Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pi¬d=S013865572003000100007
Kalita S, Kandimalla R, Sharma K, Kataki A, Deka M, Kotoky J. Amoxicillin functionali¬zed gold nanoparticles reverts MRSA resistance. Materials Science and Engineering: C. 2016;61:720-727.
Lee J, Chatterjee D, Lee M, Krishnan S. Gold nanoparticles in breast cancer treatment: Promise and potential pitfalls. Cancer Letters. 2014;347(1):46-53.
León K, Santiago J. Propiedades antimicrobianas de películas de quitosano-alcohol poli¬vinílico embebidas en extracto de sangre de grado. Revista de la Sociedad Química del Perú. 2007;173(3):158-165.
Monge M. Nanopartículas de plata: método de síntesis en disolución y propiedades bacte¬ricidas. An. Quím. 2009;105(1):33-41.
Mukherjee P, Bhattacharya R, Bone N, Lee Y, Patra C, Wang S et al. Potential therapeu¬tic application of gold nanoparticles in B-chronic lymphocytic leukemia (BCLL): enhancing apoptosis. Journal of Nanobiotechnology. 2007;5(1):4.
Nirmala Grace A, Pandian K. Antibacterial efficacy of aminoglycosidic antibiotics protected gold nanoparticles—A brief study. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2007;297(1-3):63-70.
Regiel-Futyra A, Kus-Li?kiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G et al. Development of Noncytotoxic Chitosan–Gold Nanocomposites as Efficient Antibacterial Materials. ACS Applied Materials & Interfaces. 2015;7(2):1087-1099.
Shaker M, Shaaban M. Formulation of carbapenems loaded gold nanoparticles to combat multi-antibiotic bacterial resistance: In vitro antibacterial study. International Journal of Pharmaceutics. 2017;525(1):71-84.
Sperling R, Rivera Gil P, Zhang F, Zanella M, Parak W. Biological applications of gold nanoparticles. Chemical Society Reviews. 2008;37(9):1896.
Tom R, Suryanarayanan V, Reddy P, Baskaran S, Pradeep T. Ciprofloxacin-Protected Gold Nanoparticles. Langmuir. 2004;20(5):1909-1914.
Zhou Y, Kong Y, Kundu S, Cirillo J, Liang H. Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. Journal of Nanobiotechnology. 2012;10(1):19.
Burygin G, Khlebtsov B, Shantrokha A, Dykman L, Bogatyrev V, Khlebtsov N. On the Enhanced Antibacterial Activity of Antibiotics Mixed with Gold Nanoparticles. Nanoscale Research Letters. 2009;4(8):794-801.
Fernández Riverón F, López Hernández J, Ponce Martínez LM, Machado Betarte C. Resistencia bacteriana. Rev Cub Med Mil [Internet]. 2003[acceso 25 abr 2017]; 32(1): Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pi¬d=S013865572003000100007
Kalita S, Kandimalla R, Sharma K, Kataki A, Deka M, Kotoky J. Amoxicillin functionali¬zed gold nanoparticles reverts MRSA resistance. Materials Science and Engineering: C. 2016;61:720-727.
Lee J, Chatterjee D, Lee M, Krishnan S. Gold nanoparticles in breast cancer treatment: Promise and potential pitfalls. Cancer Letters. 2014;347(1):46-53.
León K, Santiago J. Propiedades antimicrobianas de películas de quitosano-alcohol poli¬vinílico embebidas en extracto de sangre de grado. Revista de la Sociedad Química del Perú. 2007;173(3):158-165.
Monge M. Nanopartículas de plata: método de síntesis en disolución y propiedades bacte¬ricidas. An. Quím. 2009;105(1):33-41.
Mukherjee P, Bhattacharya R, Bone N, Lee Y, Patra C, Wang S et al. Potential therapeu¬tic application of gold nanoparticles in B-chronic lymphocytic leukemia (BCLL): enhancing apoptosis. Journal of Nanobiotechnology. 2007;5(1):4.
Nirmala Grace A, Pandian K. Antibacterial efficacy of aminoglycosidic antibiotics protected gold nanoparticles—A brief study. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2007;297(1-3):63-70.
Regiel-Futyra A, Kus-Li?kiewicz M, Sebastian V, Irusta S, Arruebo M, Stochel G et al. Development of Noncytotoxic Chitosan–Gold Nanocomposites as Efficient Antibacterial Materials. ACS Applied Materials & Interfaces. 2015;7(2):1087-1099.
Shaker M, Shaaban M. Formulation of carbapenems loaded gold nanoparticles to combat multi-antibiotic bacterial resistance: In vitro antibacterial study. International Journal of Pharmaceutics. 2017;525(1):71-84.
Sperling R, Rivera Gil P, Zhang F, Zanella M, Parak W. Biological applications of gold nanoparticles. Chemical Society Reviews. 2008;37(9):1896.
Tom R, Suryanarayanan V, Reddy P, Baskaran S, Pradeep T. Ciprofloxacin-Protected Gold Nanoparticles. Langmuir. 2004;20(5):1909-1914.
Zhou Y, Kong Y, Kundu S, Cirillo J, Liang H. Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guérin. Journal of Nanobiotechnology. 2012;10(1):19.
Díaz Sánchez, S., Martínez Lanao, J., & Colino Gandarillas, C. I. (2018). Incorporation of anti-infectious agents on metallic nanoparticles of gold and chitosan. FarmaJournal, 3(2), 53–61. Retrieved from https://revistas.usal.es/cinco/index.php/2445-1355/article/view/18645
Downloads
Download data is not yet available.
+
−