Study of genes involved in biofilm formation with potential involvement in the pathogenesis bacterial using Tn5mutants libraries
Abstract Biofilm formation is important in pathogenesis of a big number of bacteria. Escherichia coli, is a gram- negative bacterium, rod-shaped, facultatively anaerobic, coliform bacterium commonly found in the intestine of many animal species. Most E. coli strains are non- pathogenic, but some serotypes can cause serious diseases in their hosts. E.coli biofilm development is a complex process important for disease and engineering applications. Therefore, the description of genes implicated in the biofilm formation process is of utmost importance. Consequently, in this study we have grown a collection of 40 different mutants from the DH5? E.coli strain by using the Tn5 transposon insertion, in order to study the possible implications of the mutated genes in the biofilm formation process in this bacterium. The discovery of genes implicated in biofilm formation in E.coli may serve in future investigations of drugs against infections caused by this bacterium.
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Rodriguez E.P., Soares P, Galvao P.G, Imada E.L, Simoes-Araújo J.L, Rouws L. F. M, de Oliveira A.L, Vidal M.S, Baldani J.I. Identification of Genes Involved in Indole 3 Acetic Acid Biosynthesis by Gluconacetobacter diazotrophicus PAL5 Strain Using Transposon Mutagenesis. Front. Microbiol. 2016; 7:1572.
Smith J.L., Fratamico P.M. Escherichia coli as a Pathogen. Foodborne Diseases (Third edition). Elsevier. 2017; 189-208.
Stewart, P.S. and Costerton, J.W. Antibiotic resistance of bacteria in biofilms. The lancet. 2001; 358:135-138.
Yang R, Abraham S, Gardner GE, Ryan U, Jacobson C. Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coli O157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia. Aust Vet J. 2017; 95 (5): 143-148.
Lin Z, Dong H, Li Y. Improvement of butanol production by Escherichia coli via Tn5 transposon mediated mutagenesis. Sheng Wu Gong Cheng Xue Bao. 2015; 31(12): 1711-9.
Luo J, Qiu W, Chen L, Anjum SI, Yu M, Shan C, Ilyas M, Li B, Wang Y, Sun G. Identification of Pathogenicity-Related Genes in Biofilm-Defective Acidovorax citrulli by Transposon Tn5 Mutagenesis. Int J Mol Sci. 2015; 16(12):28050-62.
Mesarich CH, Rees-George J, Gardner PP, Ghomi FA, Gerth ML, Andersen MT, Rikkerink EH, Fineran PC, Templeton MD. Transposon insertion libraries for the characterization of mutants from the kiwifruit pathogen Pseudomonas syringae pv. actinidiae.PLoS One. 2017;12(3):e0172790.
Reisner A., Krogfelt K.A., Klein B.M., Zechner E.L., Molin S. In vitro biofilm formation of commensal and pathogenic Escherichia coli strains: impact of environmental and genetic factors. Journal of bacteriology. 2006;188(10):3572-81.
Rodriguez E.P., Soares P, Galvao P.G, Imada E.L, Simoes-Araújo J.L, Rouws L. F. M, de Oliveira A.L, Vidal M.S, Baldani J.I. Identification of Genes Involved in Indole 3 Acetic Acid Biosynthesis by Gluconacetobacter diazotrophicus PAL5 Strain Using Transposon Mutagenesis. Front. Microbiol. 2016; 7:1572.
Smith J.L., Fratamico P.M. Escherichia coli as a Pathogen. Foodborne Diseases (Third edition). Elsevier. 2017; 189-208.
Stewart, P.S. and Costerton, J.W. Antibiotic resistance of bacteria in biofilms. The lancet. 2001; 358:135-138.
Yang R, Abraham S, Gardner GE, Ryan U, Jacobson C. Prevalence and pathogen load of Campylobacter spp., Salmonella enterica and Escherichia coli O157/O145 serogroup in sheep faeces collected at sale yards and in abattoir effluent in Western Australia. Aust Vet J. 2017; 95 (5): 143-148.
Galán, I., García-Fraile, P., & Rivas, R. (2018). Study of genes involved in biofilm formation with potential involvement in the pathogenesis bacterial using Tn5mutants libraries. FarmaJournal, 3(1), 67–76. Retrieved from https://revistas.usal.es/cinco/index.php/2445-1355/article/view/17456
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