Diseño de una formulación de liposomas para la administración de vacunas

Abstract

Antigen is not the single component involved in the pharmacological response for vaccines but additional factors such as formulation and adjuvant compounds play a relevant role. Several strategies based on the use of liposomes are currently assayed in this field. According to this, the aim of the present work was the design, preparation and characterization of a pharmaceutical vehicle able to produce a rapid delivery of adjuvants and a slow release of antigen. From the know-how a theoretical vehicle was design and proposed as optimal formulation and this was then prepared and characterized. The “proposed formulation” is based on albumin coated liposomes made of egg phosphatidylcholine, cholesterol and diethyldodeccyiamonium. The applied procedure carried out in absence of organic solvents allowed for obtaining liposomes with a 20-70 nm aerodynamic diameter range and a zeta potential mean value of 61.9 ±2.08 mV and also for its coating with albumin. Coated particles showed a spherical shape and a size range of 2-10 µm which does not fulfil parenteral formulations requirements but are optimal for nasal or dermatological administration routes.