Assessment of the SmMIT-LAMP technique for the molecular detection of Schistosoma mansoni DNA in urine samples
Abstract Background: Human schistosomiasis, is one of the most common parasitic diseases worldwide. Parasitological and serological techniques have different shortcomings to control this illness. Therefore, the development of new diagnostic methods to detect infection in acute phase is required. Loop-mediated isothermal amplification technique (LAMP) could be a good choice.Methodology / Results: Firstly, experimental model was used to obtain urine samples from mice infected with cercariae of S. mansoni. The samples were collected weekly from week 0 to 8th post-infection. Finally, SmMIT-LAMP technique was performed to analyse urine samples DNA of S. mansoni was detected since 3rd week post-infectionConclusions / Significance: We have detected, for the first time in acute phase, DNA of the S. mansoni in urine samples of infected mice, using a simple, rapid, inexpensive, and potentially applicable method to the diagnosis of schistosomiasis in endemic areas.
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Fernández-Soto, P., Mvoulouga, P. O., Akue, J. P., Abán, J. L., Santiago, B. V., Sánchez, M. C., & Muro, A. Development of a highly sensitive Loop-Mediated Isothermal Amplification (LAMP) method for the detection of Loa loa. PLoS One. 2014; 9: e94664.
Fernández-Soto, P., Gandasegui Arahuetes, J., Sánchez Hernández, A., López Abán, J., Vicente Santiago, B., & Muro, A. A Loop-Mediated Isothermal Amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: A diagnostic approach in a murine model. PLoS Negl Trop Dis. 2014; 8: e3126.
Fernández-Soto, P., Tirado, V. V., Rodríguez, C. C., Pérez-Arellano, J. L., & Muro, A. Long-term frozen storage of urine samples: a trouble to get PCR results in Schistosoma spp. DNA detection. PloS One. 2013; 8: e61703.
Kurien, B. T., Everds, N. E., & Scofield, R. H. Laboratory Animals. 2004; 38: 333J361.
Notomi T, Okayama H, Masubuchi H. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28: E63.
Sandoval, N., Siles-Lucas, M., Perez-Arellano, J. L., Carranza, C., Puente, S., Lopez-Aban, J., & Muro, A. A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples. Parasitology. 2006; 133: 581-587.
Smithers SR, Terry RJ. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965; 55: 695–700.
Tomita N, Mori Y, Kanda H, Notomi. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008; 3: 877–882.
Vicente B, López-Abán J, Rojas-Caraballo J, Del Olmo E, Fernández-Soto P, Ramajo-Martín V, Muro A. The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control. Exp Parasitol. 2015; 154: 134-142.
Berhe N, Medhin G, Erko B, Smith T, Gedamu S, et al. Variations in helminth faecal egg counts in Kato-Katz thick smears and their implications in assessing infection status with Schistosoma mansoni. Acta Trop. 2004; 92: 205–212.
Cesari, I. M., & Alarcón de Noya, B. Esquistosomiasis mansoni: diagnóstico y control. Manual de campo y de laboratorio. 1987.
Doenhoff, M. J., Chiodini, P. L., & Hamilton, J. V. Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies. Trends Parasitol. 2004; 20: 35-39.
Fernández-Soto, P., Mvoulouga, P. O., Akue, J. P., Abán, J. L., Santiago, B. V., Sánchez, M. C., & Muro, A. Development of a highly sensitive Loop-Mediated Isothermal Amplification (LAMP) method for the detection of Loa loa. PLoS One. 2014; 9: e94664.
Fernández-Soto, P., Gandasegui Arahuetes, J., Sánchez Hernández, A., López Abán, J., Vicente Santiago, B., & Muro, A. A Loop-Mediated Isothermal Amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: A diagnostic approach in a murine model. PLoS Negl Trop Dis. 2014; 8: e3126.
Fernández-Soto, P., Tirado, V. V., Rodríguez, C. C., Pérez-Arellano, J. L., & Muro, A. Long-term frozen storage of urine samples: a trouble to get PCR results in Schistosoma spp. DNA detection. PloS One. 2013; 8: e61703.
Kurien, B. T., Everds, N. E., & Scofield, R. H. Laboratory Animals. 2004; 38: 333J361.
Notomi T, Okayama H, Masubuchi H. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28: E63.
Sandoval, N., Siles-Lucas, M., Perez-Arellano, J. L., Carranza, C., Puente, S., Lopez-Aban, J., & Muro, A. A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples. Parasitology. 2006; 133: 581-587.
Smithers SR, Terry RJ. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965; 55: 695–700.
Tomita N, Mori Y, Kanda H, Notomi. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008; 3: 877–882.
Vicente B, López-Abán J, Rojas-Caraballo J, Del Olmo E, Fernández-Soto P, Ramajo-Martín V, Muro A. The combination of the aliphatic diamine AA0029 in ADAD vaccination system with a recombinant fatty acid binding protein could be a good alternative for the animal schistosomiasis control. Exp Parasitol. 2015; 154: 134-142.
Martín, C., Fernandez-Soto, P., & Muro, A. (2016). Assessment of the SmMIT-LAMP technique for the molecular detection of Schistosoma mansoni DNA in urine samples. FarmaJournal, 1(1), 25–32. Retrieved from https://revistas.usal.es/cinco/index.php/2445-1355/article/view/13205
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