Avances en el desarrollo de nuevas vacunas contra Fasciola hepatica
Resumen a fasciolosis es una zoonosis provocada por el trematodo Fasciola hepatica que afecta ampliamente al sector ganadero. Sin embargo, aún no se ha desarrollado una vacuna eficaz para evitar el contagio de este parásito. En este trabajo se ha empleado la tecnología del ácido ribonucleico mensajero para desarrollar un transcrito de una proteína recombinante de Fasciola hepatica que podrá emplearse para completar la formulación de una posible vacuna. Gracias al uso de plásmidos como vectores de clonación de la secuencia de nuestra proteína antigénica de interés, en el estudio transformaremos en cultivos bacterianos dicha proteína para su posterior transcripción.
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CDC-Centers for Disease Control and Diseases. Fascioliasis. DPDx - Laboratory Identification of Parasites of Public Health Concern. 2022 [última revisión 2 mayo 2019; citado 27 junio 2022]. Disponible en: https://www.cdc.gov/dpdx/fascioliasis/index.html
Deng Z, Tian Y, Song J, An G, Yang P. mRNA Vaccines: The Dawn of a New Era of Cancer Immunotherapy. Frontiers in Immunology. 2022; 13: 1-6
Howell A, Baylis M, Smith R, Pinchbeck G, Williams D. Epidemiology and impact of Fasciola hepatica exp,osure in high-yielding dairy herds. Preventive Veterinary Medicine. 2015; 121: 41-48.
Jackson N, Kester K, Casimiro D, Gurunathan S, DeRosa F. The promise of mRNA vaccines: a biotech and industrial perspective. npj Vaccines. 2020; 11: 1-5.
López-Abán J, Casanueva P, Nogal J, Arias M, Morrondo P, Diez-Baños P et al. Progress in the development of Fasciola hepatica vaccine using recombinant fatty acid binding protein with the adjuvant adaptation system ADAD. Veterinary Parasitology. 2007; 145(3-4): 287-296.
Mas-Coma S, Valero M, Bargues M. Chapter 2 Fasciola, Lymnaeids and Human Fascioliasis, with a Global Overview on Disease Transmission, Epidemiology, Evolutionary Genetics, Molecular Epidemiology and Control. Advances in Parasitology. 2009; 69: 41-146.
Muro A, Ramajo V, López J, Simón F, Hillyer G. Fasciola hepatica: Vaccination of rabbits with native and recombinant antigens related to fatty acid binding proteins. Veterinary. 1997; 69(3-4): 219-229
Rodríguez-Pérez J, Rodríguez-Medina J, García-Blanco M, Hillyer G. Fasciola hepatica: Molecular cloning, nucleotide sequence, and expression of a gene encoding a polypeptide homologous to a Schistosoma mansoni fatty acid-binding protein. Experimental Parasitology. 1992; 74(4): 400-407.
Rong M, He B, McAllister W, Durbin R. Promoter specificity determinants of T7 RNA polymerase. Proceedings of the National Academy of Sciences. 1998; 95 (2): 515-519.
Trepotec Z, Geiger J, Plank C, Aneja M, Rudolph C. Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA. 2019; 25(4): 507-518.
Zhang Q, Honko A, Zhou J, Gong H, Downs S, Vasquez J et al. Cellular Nanosponges Inhibit SARS-CoV-2 Infectivity. Nano Letters. 2020; 20 (7): 5570-5574.
CDC-Centers for Disease Control and Diseases. Fascioliasis. DPDx - Laboratory Identification of Parasites of Public Health Concern. 2022 [última revisión 2 mayo 2019; citado 27 junio 2022]. Disponible en: https://www.cdc.gov/dpdx/fascioliasis/index.html
Deng Z, Tian Y, Song J, An G, Yang P. mRNA Vaccines: The Dawn of a New Era of Cancer Immunotherapy. Frontiers in Immunology. 2022; 13: 1-6
Howell A, Baylis M, Smith R, Pinchbeck G, Williams D. Epidemiology and impact of Fasciola hepatica exp,osure in high-yielding dairy herds. Preventive Veterinary Medicine. 2015; 121: 41-48.
Jackson N, Kester K, Casimiro D, Gurunathan S, DeRosa F. The promise of mRNA vaccines: a biotech and industrial perspective. npj Vaccines. 2020; 11: 1-5.
López-Abán J, Casanueva P, Nogal J, Arias M, Morrondo P, Diez-Baños P et al. Progress in the development of Fasciola hepatica vaccine using recombinant fatty acid binding protein with the adjuvant adaptation system ADAD. Veterinary Parasitology. 2007; 145(3-4): 287-296.
Mas-Coma S, Valero M, Bargues M. Chapter 2 Fasciola, Lymnaeids and Human Fascioliasis, with a Global Overview on Disease Transmission, Epidemiology, Evolutionary Genetics, Molecular Epidemiology and Control. Advances in Parasitology. 2009; 69: 41-146.
Muro A, Ramajo V, López J, Simón F, Hillyer G. Fasciola hepatica: Vaccination of rabbits with native and recombinant antigens related to fatty acid binding proteins. Veterinary. 1997; 69(3-4): 219-229
Rodríguez-Pérez J, Rodríguez-Medina J, García-Blanco M, Hillyer G. Fasciola hepatica: Molecular cloning, nucleotide sequence, and expression of a gene encoding a polypeptide homologous to a Schistosoma mansoni fatty acid-binding protein. Experimental Parasitology. 1992; 74(4): 400-407.
Rong M, He B, McAllister W, Durbin R. Promoter specificity determinants of T7 RNA polymerase. Proceedings of the National Academy of Sciences. 1998; 95 (2): 515-519.
Trepotec Z, Geiger J, Plank C, Aneja M, Rudolph C. Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA. 2019; 25(4): 507-518.
Zhang Q, Honko A, Zhou J, Gong H, Downs S, Vasquez J et al. Cellular Nanosponges Inhibit SARS-CoV-2 Infectivity. Nano Letters. 2020; 20 (7): 5570-5574.
Perallón Mantas, D., Sánchez Montejo, J., & Muro Álvarez, A. (2023). Avances en el desarrollo de nuevas vacunas contra Fasciola hepatica. FarmaJournal, 8(1), 49–57. https://doi.org/10.14201/fj2023814957
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