Nasopharyngeal swab for the diagnosis of COVID-19
Abstract Introduction and objective: The diagnostic test of COVID-19 with the highest sensitivity is a nasopharyngeal sample using a swab for subsequent study using RT-PCR. The objective of our work is to explain in detail the most appropriate way to perform this technique. Method: Description of the different steps necessary for taking a nasopharyngeal sample in patients with covid-19. Results: The steps to take into account to get a nasopharyngeal sample in a patient with COVID-19 are: 1- Previously, it is necessary to rule out that there are no contraindications for sampling2. Sample labeling and preparation of the petition form. 3. Placement of PPE according to the institution rules4. Explanation to the patient of the process to be performed 5. Procedure for taking a nasopharyngeal sample. &. Withdrawal of the EPI. /. Handling and tranportation of the sample to the laboratory. Discussion/Conclusions: Given that the sensitivity of the nasopharyngeal simples for the diagnosis of COVID-19 depends largely on a correct technique, the adequate training of the personnel involved in this collection is very important. The examiner must know the objetive of the procedure, how top ut on and take off the PPE, know how the technique is performed and how the sample is handled.
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5. Wu J, Liu J, Li S, et al. Detection and analysis of nucleic acid in various biological samples of COVID-19 patients [published online ahead of print, 2020 Apr 18]. Travel Med Infect Dis. 2020;101673. doi:10.1016/j.tmaid.2020.101673. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165102/. [Citado el 27/05/2020].
6. Marty FM, Chen K, Verrill KA. How to Obtain a Nasopharyngeal Swab Specimen [published online ahead of print, 2020 Apr 17]. N Engl J Med. 2020;10.1056/NEJMvcm2010260. doi:10.1056/NEJMvcm2010260. Disponible en: https://www.nejm.org/doi/full/10.1056/NEJMvcm2010260?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed. [Citado el 27/05/2020].
7. NEJM procedure: collection of nasopharyngeal specimens with the swab technique. https://www.youtube.com/watch?v=DVJNWefmHjE
8. Centers for Disease Control and Prevention. Interim guidelines for collecting, handling, and testing clinical specimens from persons for coronavirus disease 2019 (COVID-19). April 14, 2020. Disponible en: https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html. [Citado el 27/05/2020].
9. World Health Organization. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases: interim guidance. March 2, 2020 Disponible en: https://apps.who.int/iris/handle/10665/331329. [Citado el 27/05/2020].
Morales-Angulo, C., González-Zubizarreta, R., Martín-Toca, G., Ramírez-Bonilla, A., Gonzalo-Margüello, M., & Rodríguez-Fernández, A. (2020). Nasopharyngeal swab for the diagnosis of COVID-19. Revista ORL, 11(4), 389–394. https://doi.org/10.14201/orl.23079
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